An overview of CMIF instruments and confocal training seminar will be presented Tuesday, March 28th, 2023 from 1:30-3:30pm in BRT 115. Please register at Contact-Uswith a comment "CMIF Training Seminar". Please note that you will be registered, but should not expect to receive a confirmation.
An overview of CMIF instruments and confocal training seminar was presented previously. View PDF of presentation slides. Presentation slides can be accessed by mobile devices with the below QR code:
Learn how to enhance your research with the Talos L120C TEM microscope! Featuring Dr. Natalia de Val, Thermo Fisher Scientific
Click here to register!
The Talos L120C, provides proven optimal imaging performance on a platform designed for modular versatility, maximum stability and operational ease. It is suitable for multi-user environments supporting applications from negative stain electron microscopy (pathology samples), Single Particle Analysis (SPA), micro-electron diffraction (micro-ED) and tomography.
Hosted by the OSU CCC-James Microscopy Shared Resource
Giovanna Grandinetti and Sarah Mikula at the Center for Electron Microscopy and Analysis (CEMAS) and Jeff Tonniges at the Campus Microscopy and Imaging Facility (CMIF) received a Staff Career Development grant to develop a correlative light and electron microscopy (CLEM) pipeline at The Ohio State University. The grant is to develop “Cross-Facility Collaboration for the Development of On-Site Correlative Light and Electron Microscopy Imaging Techniques.”
“This grant will help the Ohio State research community by bringing access to an advanced microscopy technique directly to researchers through core facilities right here at The Ohio State University,” said Mikula, laboratory supervisor and principal investigator (PI) at CEMAS. “It will also increase collaborative research between our two microscopy facilities, which is beneficial to researchers across the university and beyond.”
Correlative light and electron microscopy (CLEM) is a method by which a sample is imaged using light or fluorescence microscopy to find a protein or area of interest, then subsequently imaged using high-resolution electron microscopy on the same area. Combining both techniques allows researchers to acquire more information than using each technique individually and to determine answers to research questions they could not otherwise obtain.
Some potential uses for CLEM include studying virus or nanoparticle-treated cells to gain high resolution information on intracellular trafficking or observing specific protein-protein interactions on the cytoskeleton. The Ohio State University currently has state-of-the-art light microscopy capabilities housed at CMIF and world-class electron microscopy capabilities housed predominantly at CEMAS, however a CLEM workflow on campus had yet to be established.
CLEM allows fully hydrated and potentially live cell specimens to be examined by confocal or multiphoton microscopy, then subsequently prepared for examination for further imaging using electron microscopy. CLEM is applicable for both scanning electron microscopy (SEM) and transmission electron microscopy (TEM).
This development of a CLEM workflow at Ohio State is a collaborative effort between CEMAS and CMIF. Creating a streamlined workflow capitalizes on the individual strengths of the two core facilities and sets the foundation for future collaborations. Sadie Potts, a Student Research Assistant at CMIF, is currently helping with building these workflows.
“CMIF and CEMAS combined serve OSU’s Comprehensive Cancer Center Microscopy Shared Resource (MSR), funded by the National Institutes of Health, so it is very exciting to collaborate on this project together,” said Tonniges, senior research associate at CMIF. “We look forward to continuing our collaborations in the future.”
For more information about how microscopy services can positively impact your research, please reach out to CCC_Microscopy@osu.edu.
Confocal and transmission electron microscopy of brain tissue imaged by CLEM technique. Image from S. Modla and K.J. Czymmek, Correlated Microscopy: a powerful tool for exploring neurological cells and tissues.
Join us Dec. 14 to learn how you can use cryogenic-electron microscopy (cryo-EM) to accelerate your research. The Center for Electron Microscopy and Analysis (CEMAS) is hosting a “Getting Started with Cryo-EM” event where we connect the needs of biomedical researchers with what is possible using our advanced instrumentation. There will be a general overview covering the wide variety of cryo-EM applications followed by two sessions that highlight exciting work being carried out by our users. Informal lunch and CEMAS tours to follow.
Ticks are external parasites. They are widely distributed around the world, especially in warm, humid environment. Ticks live by sucking the blood as their nutrition. Because of their blood-ingesting diets and infections caused by pathogens, ticks act as vectors of many serious diseases that affect humans and other animals.
Dr. Tong Zhang imaged this sample by taking the advantage of sample auto-fluorescence under different wavelengths with a 10x objective on a Nikon A1R Confocal Microscope at CMIF. There were 423 Z-direction images covering through a 211um thickness. Extended focus function re-built the 3D Z-stack images into a 2D image with 3D information. The microscope resonant scanner boosted the acquisition speed significantly.
This image had very fine details and striking looking. The auto-fluorescence revealed tick head’s anatomy and specially the mouth region with inverted-arrow-like structure. Ticks use this kind of structure to anchor them on animals. It is a good example of nature smart designs and illustrates how our live cell imaging system can be used for lower power large field imaging in addition to high-speed 3D imaging.
https://www.nikonsmallworld.com/galleries/2021-photomicrography-competi…
