RATES:
Please refer to our rates page.
INFORMATION:
The Nikon Super-resolution Microscope system is installed in the renovated BSL2 space on the 2nd floor of the Biomedical Research Tower (BRT) at Campus Microscopy and Imaging Facility (CMIF) and Microscopy Shared Resource (MSR). The system consists of Structured Illumination Microscopy (SIM), STochastic Optical Reconstruction Microscopy (STORM) and Total Internal Reflection Fluorescence (TIRF) microscopy capabilities. The SIM and TIRF can be used with a range of commonly used fluorophores including expressed fluorescent proteins. The SIM can resolve up to 115nm in 3D-SIM mode and 86nm in TIRF-SIM mode in the X/Y plane. The STORM offers down to 20nm resolution in the X/Y plane and 50nm in the Z plane, but it requires specialized probes and optimization. This is a Widefield based system with two cameras set up for red and green imaging. This is a $750,000 instrument, which was funded by the NIH S10 grant, with supporting funds from the OSU Comprehensive Cancer Center (OSUCCC) for the STORM capability and the Office of Research for the room remodeling. The system can also be utilized as a state-of-the-art widefield imaging system for live and fixed cells and tissues.
We have strived to make the system with affordable rate at $40/hr, which is lower than the average rate for comparable Big 10 and regional academic institution microscopy cores. Dr. Anthony Vetter from Nikon has agreed to help with advanced training and to discuss applications and operations on this system. Please let us know if you would like to take advantage of this opportunity. The CMIF/MSR staff are happy to provide tours and trainings as well.
We hope to see you soon and help your advancement in research with our services.
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