Transmission Electron Microscope for biological research located in the basement of the BRT, room 080B.
JEOL 120i TEM
High-contrast polepiece for optimal imaging of biological specimens
Room temperature samples only
Adjustable accelerating voltage from 20-120 kV for optimizing imaging conditions
Magnification range: 50-1,200,000 X
Lattice line resolution limit: down to 0.2 nm
AMT NanoSprint 15L-B camera - low-noise sCMOS sensor offers a large 15 megapixel sampling region with a high speed 30fps readout
Funding Recognition
This microscope was obtained through funds from Enterprise for Research, Innovation and Knowledge (ERIK).
Microscope
JEOL JEM-120i
Accelerating Voltage
20-120kV
Magnification
50-1,200,000X magnification
Resolution
0.2 nm (lattice)
0.38 nm (point)
Stage Control
Ambient (20° C) operating temperature
High tilt single specimen holder for room temperature tomography with an alpha tilt ±80°
Optical System
NanoSprint15B: High Sensitivity sCMOS TEM Camera
AMT Image Capture Software version 702.176
16-bit digitization
15 megapixels
30 fps
8.5 x 8.5 µm phosphor pixel size
5056 x 2960 pixel sensor size
Serial EM
Assists with automated EM data acquisition.
Acquire tilt series images for tomography
Acquire large area images for 3-D reconstructions of serial images
Assists with correlative light and electron microscopy
Note
For other electron microscopy services (e.g., aberration-corrected high-resolution imaging, chemical analysis, cryo-electron microscopy), please contact the Center for Electron Microscopy and Analysis.