System Overview
This advanced system integrates three powerful imaging technologies:
- Structured Illumination Microscopy (SIM)
- Live and fixed samples up to 10 um thickness
- STochastic Optical Reconstruction Microscopy (STORM)
- For fixed samples and requires specialized fluorophores and imaging buffer
- Total Internal Reflection Fluorescence (TIRF) microscopy
- Observe bottom 100-200 nm of living cells
- Single molecule studies
- Highly Inclined and Laminated Optical sheet (HILO) microscopy capabilities
- The system features dual-camera setup for simultaneous red and green channel imaging, with exceptional resolution capabilities.
Access and Support
The Nikon Super-Resolution Imaging System is available for independent use. Before beginning your imaging project, please EMAIL the CMIF staff with a detailed description of your experiment. We will be able to provide you with advice on how to prepare your samples for optimal results. Training is performed on an individual basis using the researchers’ samples and instrument rates apply.
BSL-2 training from EHS is required for all researchers wishing to perform live cell imaging at the CMIF. A signed form must be submitted to the facility before the start of any live cell imaging project.
- Advanced training available through Nikon
- CMIF/MSR team provides tours and training
Funding Recognition
This system and capabilities were established through:
- NIH S10 grant (primary funding)
- Ohio State University Comprehensive Cancer Center (STORM capability)
- Enterprise for Research, Innovation and Knowledge (room renovation)
