Campus Microscopy and Imaging Facility

Nikon AXR Confocal Microscope

An advanced confocal microscope designed for both live and fixed cell imaging. Located in room 0022 of the Pelotonia Research Center

The Nikon AXR confocal microscope is a newly released upgrade to the A1R series.

  • Inverted point-scanning confocal with resonant and galvano scanners
  • Available for both live and fixed samples including, tissue, organoids, cultured cells, bacteria
  • Available for time-lapse imaging, FRAP, confocal reflectance microscopy, spectral imaging
  • Accepts slides, multi-well plates, 35 mm and 60 mm dishes
  • Live-cell incubation chamber
  • Equipped with objectives from 2x/0.10 up to 60x/1.42, 4 fluorescence detectors, and 4 diode-pumped solid-state lasers ranging from 405-640 nm
  • 60X water-immersion objective available upon request

In comparison to the A1R capabilities, the AXR allows:

  • Faster scanning capabilities for quicker imaging times and reduced photobleaching
  • Larger scanhead for collection of larger fields of view (FOV)
  • Up to 8K x 8K resolution for Galvano Scanner (2x the A1R)
  • Up to 2K x 2K resolution for Resonant Scanner (2x the A1R)
  • Piezo drive capable of capturing 600 um z-stacks (6x the A1R)

Please note, the AXR is located in room 0022 of the Pelotonia Research Center in the West Campus Carmenton district, not in the BRT.

Access and Support

The Nikon AXR Live Cell Imaging Confocal Microscope is available for independent use. Before beginning your live cell imaging project, please EMAIL the CMIF staff with a detailed description of your experiment. We will be able to provide you with advice on how to prepare your samples for optimal live cell imaging results. Training is performed on an individual basis using the researchers’ samples and instrument rates apply.

BSL-2 training from EHS is required for all researchers wishing to perform live cell imaging at the CMIF. A signed form must be submitted to the facility before the start of any live cell imaging project.