Campus Microscopy and Imaging Facility

FEI Tecnai G2 Spirit TEM

Transmission Electron Microscope for Biological Research at Ohio State's CMIF. Located in BRT 245G.

Transmission Electron Microscope

  • FEI Tecnai G2 Spirit BioTwin TEM (80kV).
  • High contrast polepiece and low kV operating voltage, ideal for imaging biological samples
  • Room temperature samples only
  • Full service TEM sample preparation and imaging available upon request

Note

For other electron microscopy services (e.g., aberration-corrected high-resolution imaging, chemical analysis, cryo-electron microscopy), please contact the Center for Electron Microscopy and Analysis.

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Sample Images
Gallery Image
A transmission electron microscope (TEM) setup in a laboratory setting. The microscope consists of a tall vertical column in metallic silver color, with various mechanical controls and apertures visible. Two computer monitors are positioned on a desk next to the microscope for digital image viewing and instrument control. The setup appears to be in a typical research laboratory environment with ceiling tiles visible above.
A black and white transmission electron microscope (TEM) image showing a cellular or tissue cross-section. The image reveals a network pattern of dark walls or membranes surrounding lighter-colored, irregular polygonal spaces of varying sizes. The structure resembles a honeycomb or plant cell architecture where the spaces appear white and the boundaries appear black.
A black and white transmission electron microscope (TEM) image showing what appears to be the inverse or negative of the previous image. Here, the network pattern appears as white walls or membranes against a black background, creating interconnected pathways surrounding dark, irregular polygonal spaces. The structure has a web-like or mesh appearance with varying sizes of openings.
A transmission electron microscope (TEM) image showing what appears to be a bacterial cell with multiple flagella. The image is in black and white, with the bacterium appearing as a dark oval shape against a lighter background. Multiple wavy, thread-like flagella extend from the bacterial cell body in various directions, giving it a characteristic whip-like appearance. The image has a grainy texture typical of TEM microscopy.
A transmission electron microscope (TEM) image showing complex cellular ultrastructure. The image is in black and white with varying shades of gray, revealing intricate membrane structures and organelles. The structures appear branched or folded, with distinct dark and light regions creating a complex pattern. The image shows fine detail of what appears to be either cell membranes or tissue interfaces with various cellular components visible within the structure.
A transmission electron microscope (TEM) image showing a cross-section of what appears to be a white blood cell or leukocyte. The cell is roughly circular and contains multiple dark-staining nuclei (likely 3-4 visible) characteristic of polymorphonuclear cells. The nuclei appear as distinct lobed structures within the cell, and the cytoplasm contains numerous small granules giving it a speckled appearance. The image is in black and white with the cell structure clearly visible against a lighter background.
Carousel Description
These transmission electron microscope (TEM) images showcase various biological structures at extremely high magnification, demonstrating the power and versatility of electron microscopy in biological research. The collection includes views of different cellular and subcellular structures: a bacterial cell with its characteristic flagella, a polymorphonuclear white blood cell with its distinctive multi-lobed nucleus, complex membrane structures showing cellular ultrastructure, and what appears to be plant or tissue cross-sections showing honeycomb-like patterns in both positive and negative contrast. The first image shows the TEM instrument itself - a sophisticated piece of equipment featuring a tall column and dual monitors for digital image acquisition and analysis. Together, these images illustrate how TEM enables researchers to visualize biological structures at the nanoscale level, revealing details that would be impossible to see with conventional light microscopy.
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