Campus Microscopy and Imaging Facility

Zeiss Axioskop Microscope

Brightfield, polarized light, and widefield fluorescence capabilities. Located in BRT 245K.

Zeiss Axioskop Microscope 

  • Available for capturing brightfield, darkfield, phase contrast, polarized light, and widefield fluorescence images
  • QICLICK-F-M-12 CCD camera
  • 4 filter sets: Cy5, Rhodamine, FITC, DAPI
  • Manual stage

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Sample Images
Gallery Image
Microscopic image of stained tissue section showing red-stained muscle fibers or blood vessels interspersed with blue/purple connective tissue. The image demonstrates clear tissue architecture and cellular organization typical of histological preparations.
Phase contrast microscopy image showing individual cells with visible cellular structures. The grayscale image reveals clear detail of cytoplasmic granules, vacuoles, and nuclei within the cells. The cells appear to be epithelial or cultured cells with distinct cell boundaries and internal organelles visible due to the phase contrast technique.
Four-panel fluorescence microscopy image showing the same cells labeled with different fluorescent markers. Top left shows Rhodamine (red) fluorescence, top right shows FITC (green) fluorescence, bottom left shows Cy5 (far-red, displayed in cyan) fluorescence, and bottom right shows the merged composite of all channels. Each panel captures three cell nuclei or cellular structures with distinct fluorescent labeling patterns. Each image is labeled underneath with its corresponding fluorescent marker and 'cube
Carousel Description
The image suite demonstrates the versatility of fluorescence microscopy techniques in visualizing cellular structures and molecular targets. The first image showcases actin filament organization, combining three fluorescent channels to reveal cytoskeletal architecture (green), nuclear positioning (blue), and an additional cellular marker (red). This is followed by a series of four related panels demonstrating multichannel imaging capabilities using different fluorescent markers - Rhodamine (red), FITC (green), and Cy5 (displayed in cyan) - with the fourth panel showing how these channels can be merged to create a composite view. This type of imaging approach allows researchers to simultaneously visualize multiple cellular components or molecular targets within the same sample, providing valuable insights into their spatial relationships and potential interactions. The clarity and specificity of the fluorescent labeling, combined with the high-contrast dark background, enables detailed examination of cellular structures and their organization within the tissue or culture environment.
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