Campus Microscopy and Imaging Facility

New Light Sheet Microscope and Introduction Seminar!

January 21, 2026

The Campus Microscopy and Imaging Facility (CMIF) and the Center for Brain Injury Recovery and Discovery (formerly, Chronic Brain Injury Program) are happy to announce the acquisition of a Miltenyi UltraMicroscope Blaze Light Sheet Microscope housed in Room 245A of CMIF in the Biomedical Research Tower (BRT). The new light sheet microscope is installed and ready to use. This scope is designed for large, cleared samples. 

CMIF will be hosting a seminar titled "Introduction to Tissue Clearing and Light Sheet Imaging for 3D Biology using the Miltenyi Blaze" on February 26th from 1-2 pm in BRT Room 480. You can also join virtually by clicking here for the Zoom link.

Please join us for a discussion on methods for 3D visualization of intact, cleared tissue samples using the Ultra Microscope Blaze. No prior experience is necessary.

We will review the fundamental principles of light-sheet microscopy, with a focus on operating the Ultra Microscope Blaze. 

The seminar will also cover different classes of tissue clearing, highlighting the advantages and limitations of each, and providing practical examples of 3D imaging in research. The presentation will conclude with a Question-and-Answer session.

Attendees are welcome to reach out after the seminar for tissue-clearing consultations and application-specific questions.

Click here to RSVP. 

Feel free to submit questions beforehand by emailing cmif@osu.edu. 
 

Miltenyi UltraMicroscope Blaze Light Sheet Microscope

Introducing the Miltenyi UltraMicroscope Blaze Light Sheet Microscope 

The Blaze is ideal for large, cleared samples. 

The main benefits are:

  • Multiple light sheets optimized for uniform illumination
  • Automated features for smooth transition between samples and objective switching
  • Sample holders tailored to your needs, including chambers to image multiple rodent organs or organoids up to an entire mouse
  • Easy adaptation to different clearing solutions
  • High-resolution to view sub-cellular details

The specifications are as follows:

  • Light sheet thickness: <4.5 μm @488 nm
  • Focus range: >±5mm from center position of sample for all imaging solutions
  • Motorized detection module for up to three MI Plan objective lenses
    • Motorized functions: z-drive, autofocus, filter wheel for up to seven 43 mm emission
  • Objective lenses of 1.1x, 4x, and 12x
  • magnification change unit (0.6x,1x,1.67x and 2.5x), so magnification from 0.66x to 30x
  • Motorized XYZ-stage for moving the cuvette and the sample holder (travel range 24 x

                   50 x 23 mm xyz)

  • A motorized cuvette release enables easy sample access
  • Standard Cuvette with 497 ml capacity and a XXL Chamber with 715 ml capacity
  • 4.2 Mpx camera:
    • 2048 x 2048 pixels
    • 6.5 μm pixel size
    • QE: 82% @Peak
  • Laser Beam Combiner equipped with following laser modules and emission filters:
    • 405 nm  75 mW, EMF460/40
    • 488 nm  75 mW, EMF525/50
    • 561 nm  75 mW, EMF620/60
    • 640 nm  65 mW, EMF680/30
    • 785 nm  75 mW, EMF805LP

 

Funding for the Miltenyi UltraMicroscope Blaze was provided by the Center for Brain Injury Recovery & Discovery (formerly, Chronic Brain Injury Program).